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Catalog Number | orb555858 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against MICA conjugated to PE |
Clonality | Monoclonal |
Clone Number | 6D4 |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | Transfected C1R cells expressing MICA |
Concentration | 0.1 mg/ml |
Dilution range | Flow cytometry: Recommended dilution: 1-5 μg/ml. |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | MICA/MICB |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Note | For research use only |
Application notes | Flow cytometry: Recommended dilution: 1-5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Separation of HeLa cells (red-filled) stained using anti-MICA/MICB (6D4) PE antibody (concentration in sample 0.56 μg/ml) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 0.56 μg/ml, same as anti-MICA/MICB PE concentration, black-dashed) in flow cytometry analysis (surface staining).
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