MICA Antibody

• Catalog Number: orb2634920
• Category: Antibodies
• Description: MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with γδ T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MICA/4442
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 1-200 was used as the immunogen for the MICA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MICA antibody is available for research use only.
• UniProt ID: Q29983
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the MICA antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human stomach with MICA antibody (clone MICA/4442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with MICA antibody (clone MICA/4442). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MICA antibody (clone MICA/4442) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MICA antibody (clone MICA/4442). These results demonstrate the foremost specificity of the MICA/4442 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.