MHC II Antibody [P7/7], Rabbit IgG

• Catalog Number: orb1671843
• Category: Antibodies
• Description: MHC II Antibody [P7/7], Rabbit IgG
• Clonality: Recombinant
• Clone Number: P7/7
• Tested applications: FC, IHC, IP
• Reactivity: Mouse, Rat
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-MHC II antibody P7/7. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r1 version of P7/7 (orb1671843 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-MHC II antibody (P7/7). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of P7/7 (orb1671843) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671843- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.