MHC I Antibody [R1-21.2], Rabbit IgG

• Catalog Number: orb1671839
• Category: Antibodies
• Description: MHC I Antibody [R1-21.2], Rabbit IgG
• Clonality: Recombinant
• Clone Number: R1-21.2
• Tested applications: IHC, IP, RIA
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: R1.21.2, H-2, MHC-I, MHC Class I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of mouse splenocytes using anti-MHC I antibody (R1-21.2). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of R1-21.2 (orb1671839) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671839- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-MHC I antibody R1-21.2. Mouse thymus tissue lysate (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of R1-21.2 (orb1671839) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.