MGMT Antibody

• Catalog Number: orb334008
• Category: Antibodies
• Description: Mouse monoclonal antibody to MGMT
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A11F8
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 21.6kDa
• Target: MGMT
• Entrez: 4255
• UniProt ID: P16455
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: EC 2.1.1.63
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Rodriguez, Fausto J. et al. MGMT immunohistochemical expression and promoter methylation in human glioblastoma Appl Immunohistochem Mol Morphol, 16, 59-65 (2008)

PubMed: 18091318

Immunofluorescence analysis of Hela cells using MGMT antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using MGMT antibody

Western blot analysis of human recombinant protein using MGMT antibody

Western blot analysis of HEK293 using MGMT antibody

Western blot analysis of MCF-7 (Lane1), Jurkat (Lane2), HepG2 (Lane3), and PC-3 (Lane4) cell lysate using MGMT antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Hela cells using MGMT antibody