MFF Antibody

• Catalog Number: orb1520707
• Category: Antibodies
• Description: MFF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 50 and 100
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MFF
• UniProt ID: Q9GZY8
• NCBI: Q9GZY8.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: C2orf33; mitochondrial fission factor; EMPF2; GL00
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse MFF by western blot. Samples: Whole cell lysate from HeLa (5 and 15 µg) 293T, (50µg) , and Jurkat (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MFF antibody (orb1520707) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50µg) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MFF antibody (orb1520707) used for IP at 2 µg per reaction.