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Catalog Number | orb1255879 |
---|---|
Category | Antibodies |
Description | METTL3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 80kDa |
Target | METTL3 |
UniProt ID | Q86U44 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | METTL3, M6A, IME4, Spo8, MT-A70 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using METTL3 antibody (orb1255879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.
Immunofluorescence analysis of C6 cells using METTL3 antibody (orb1255879) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using METTL3 antibody (orb1255879) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using METTL3 antibody (orb1255879) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 150 ug extracts of 293T cells using 3 ug METTL3 antibody (orb1255879). Western blot was performed from the immunoprecipitate using METTL3 antibody (orb1255879) at a dilution of 1:1000.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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