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Catalog Number | orb2276572 |
---|---|
Category | Antibodies |
Description | Mesothelin antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | Biorbyt-235M |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary) |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | Q13421 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | CAK1; Megakaryocyte potentiating factor; Mesotheli Read more... |
Note | For research use only |
Application notes | Positive Control: Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells. Negative Control: Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining. Cellular Localization: Cell Surface and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Breast Adenoid cystic breast cancer with strong membranous mesothelin immunostaining in a subset of cells.
Ovary Strong predominantly membranous mesothelin staining in an endometroid ovarian cancer.
Stomach Strong predominantly cytoplasmic mesothelin immunostaining in a stomach cancer with diffuse growth pattern.
IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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