Mesothelin antibody

• Catalog Number: orb2276570
• Category: Antibodies
• Description: Mesothelin antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-235M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2b, kappa
• Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q13421
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: CAK1; Megakaryocyte potentiating factor; Mesotheli
• Note: For research use only
• Application notes: Positive Control: Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells. Negative Control: Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining. Cellular Localization: Cell Surface and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Breast Adenoid cystic breast cancer with strong membranous mesothelin immunostaining in a subset of cells.

Ovary Strong predominantly membranous mesothelin staining in an endometroid ovarian cancer.

Stomach Strong predominantly cytoplasmic mesothelin immunostaining in a stomach cancer with diffuse growth pattern.