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Catalog Number | orb1825302 |
---|---|
Category | Antibodies |
Description | Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | MSLN/8391R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant partial protein sequence (within amino acids 273-407) from the human protein was used as the immunogen for the recombinant Mesothelin antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant Mesothelin antibody is available for research use only. |
UniProt ID | Q13421 |
Storage | Aliquot the recombinant Mesothelin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human ovarian cancer tissue with recombinant Mesothelin antibody (MSLN/8391R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Mesothelin antibody (clone MSLN/8391R) as confirmation of integrity and purity.
IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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