Membrane type 1 metalloprotease Antibody [LEM-2/15], Mouse IgG1

• Catalog Number: orb1672033
• Category: Antibodies
• Description: Membrane type 1 metalloprotease Antibody [LEM-2/15], Mouse IgG1
• Clonality: Recombinant
• Clone Number: LEM-2/15
• Tested applications: Blocking, ELISA
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: The cyclic peptide possessing the sequence of residues 218-233 of the V-B-loop of MT1-MMP.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: MT1-MMP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-membrane type 1 metalloprotease antibody LEM-2/15. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of LEM-2/15 (orb1672033) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing nuclear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672033- DAPI- merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-MT1-MMP (Membrane type 1 metalloprotease) antibody LEM-2/15. A549 (A)- HepG2 (B) and HeLa (C) cell lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LEM-2/15 (orb1672033) at 0.3 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unprocessed MT1-MMP is 65.9 kDa. A signal peptide and propeptide are cleaved from MT1-MMP during post-translational processing. The processed form of MT1-MMP may be seen in all samples- and the unprocessed form in lanes B and C. orb1672033 successfully detected MT1-MMP in A549- HepG2 and HeLa cell lysates.