Melan A CR (Cross Reactive) antibody

• Catalog Number: orb2276539
• Category: Antibodies
• Description: Melan A antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-901M+
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-length hMART-1 protein
• Dilution range: 1:50-1:100
• Conjugation: Unconjugated
• UniProt ID: Q16655
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen
• Note: For research use only
• Application notes: Positive Control: Skin: Virtually all melanocytes should show a strong Melan A+ immunostaining including a weak to moderate staining in melanocytic dendrites. Adrenal gland: An at least moderate Melan A+ immunostaining should be seen in adrenocortical cells. Negative Control: Kidney: Melan A immunostaining should be absent in all cells. Adrenal gland: Melan A+ immunostaining should be absent in all medullary cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:75 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Adrenal gland Adrenocortical adenoma showing diffuse strong Melan A positivity.

Kidney Clear cell renal cell carcinoma showing moderate to strong Melan A immunostaining of tumor cells.

Skin Malignant melanoma with strong Melan A positivity in all tumor cells.