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MEK1 C-Term Antibody Biotin
Catalog Number: orb420266
| Catalog Number | orb420266 |
|---|---|
| Category | Antibodies |
| Description | MEK1 antibody (Biotin) |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 13B6.G12 |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH. |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:40,000, WB: 1µg/mL |
| Form/Appearance | Lyophilized |
| Purity | This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1. Anti-MEK1 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform. |
| Conjugation | Biotin |
| UniProt ID | Q02750 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | mouse anti-MEK1 antibody biotin conjugation, bioti Read more... |
| Note | For research use only |
| Application notes | Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Anti-MEK1 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50 ng per lane. Primary Antibody: Anti-MEK1 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40000 dilution.
