MED9 Antibody

• Catalog Number: orb402593
• Category: Antibodies
• Description: MED9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human MED9 recombinant protein (Position: A55-E146).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 19 kDa
• UniProt ID: Q9NWA0
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mediator of RNA polymerase II transcription subuni
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of MCF-7 cells using anti-MED9 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MED9 using anti-MED9 antibody.Lane 1:rat brain tissue;2:rat heart tissue;3:rat kidney tissue;4:mouse brain tissue;5:mouse heart tissue;6:mouse kidney tissue.

IF analysis of MED9 using anti-MED9 antibody. MED9 was detected in immunocytochemical section of U20S cells.

IHC analysis of MED9 using anti-MED9 antibody.MED9 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of MED9 using anti-MED9 antibody.MED9 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of MED9 using anti-MED9 antibody.MED9 was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of MED9 using anti-MED9 antibody.MED9 was detected in paraffin-embedded section of rat small intestine tissue.

IHC analysis of MED9 using anti-MED9 antibody.MED9 was detected in paraffin-embedded section of human mammary cancer tissue.