MECT1 antibody

• Catalog Number: orb345552
• Category: Antibodies
• Description: MECT1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Fish, Human, Mouse, Rat, Zebrafish
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-34 of human MECT1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human MECT1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with MECT1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q6UUV9
• NCBI: 148596965
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MECT1 antibody, MECT-1, MECT 1, TORC1,
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80-95 kDa in size corresponding to MECT1 protein by western blotting in the appropriate cell lysate or extract. This antibody is will react with isoform 1 and 2 of MECT1.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-MECT1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The working dilution is 1:19000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate stabilizer, Goat Anti-Rabbit HRP conjugated (p/n orb347654) and TMB substrate.

Western blot using Biorbyt's affinity purified anti-MECT1 antibody shows detection of endogenous MECT1 (lower arrowhead) and MECT1-MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT1 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT1 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000.