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Catalog Number | orb767440 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ME3 |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human ME3. AA range:545-594 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 67 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-ME3 antibody, anti-NADP-dependent malic enzym Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from RAW264.7 and NIH/3T3 cells, using ME3 Antibody. The lane on the right is blocked with the synthesized peptide.