MDM2 Antibody

• Catalog Number: orb1253688
• Category: Antibodies
• Description: MDM2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Immunogen: MDM2 (phospho-Ser166) antibody was raised against a peptide sequence around phosphorylation site of Serine 166 (A-I-S (p) -E-T) derived from Human MDM2.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 90 kDa
• Target: MDM2
• UniProt ID: Q00987
• NCBI: NP_002383.2
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: HDMX, hdm2, ACTFS, ESA1, M17S1,
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from 293 cells untreated or treated with Hydroxyurea using MDM2 (phospho-Ser166).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MDM2 (phospho-Ser166).