MCP1 antibody

• Catalog Number: orb345137
• Category: Antibodies
• Description: MCP1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, WB: 1:1000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human MCP-1 protein. A BLAST analysis was used to suggest cross-reactivity with MCP-1 from human sources based on 100% homology with the immunizing sequence. Based on 98 to 100% homology, there is a chance of cross-reactivity to MCP-1 from chimpanzee, orangutan, macaque, and baboon. Cross-reactivity with MCP-1 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P13500
• NCBI: NP_002973.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-MCP-1 Antibody, CCL2, C-C motif chemok
• Note: For research use only
• Application notes: This purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11,025 Da in size corresponding to the mature human MCP-1 protein by western blotting in appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, expect ~8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Biorbyt's Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20000 in blocking buffer (p/n orb348637) for 30 min.