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Catalog Number | orb344654 |
---|---|
Category | Antibodies |
Description | MCP1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:4,500, WB: 1 µg/ml |
Form/Appearance | Lyophilized |
Purity | This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Sus scrofa 85.5% and Bos Taurus 81.1%. Cross-reactivity with CCL2 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | A5PJF7 |
NCBI | 27806711 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-CCL2 antibody, CCL-2, CCL 2, Chemokine Read more... |
Note | For research use only |
Application notes | This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kDa in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8 kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with Biorbyt Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Monkey, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC-P | |
Canine, Equine, Mouse, Porcine, Rabbit | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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