MCM4 (phospho-S54) antibody

• Catalog Number: orb214235
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MCM4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S54 of human MCM4 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MCM4
• Entrez: 4173, 17217
• UniProt ID: P33991, P49717
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti CDC21 antibody, anti DNA replication licensin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MCM4 (Phospho-S54) expression in U2OS (A), rat testis (B), rat heart (C) whole cell lysates. (Predicted band size: 96 kD; Observed band size: 100 kD)

Immunohistochemical analysis of MCM4 (Phospho-S54) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.