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Catalog Number | orb1529687 |
---|---|
Category | Antibodies |
Description | MCM2, Phospho (S53) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Surrounding serine 53 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:1,000 - 1:2,500; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MCM2, Phospho (S53) |
UniProt ID | P49736 |
NCBI | NP_004517.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | D3S3194; MITOTIN; minichromosome maintenance prote Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho MCM2 (S53) by western blot.Samples: Whole cell lysate from asynchronous HEK293T cells. Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody orb1529687 used at 0.1 µg/ml.
Detection of human and mouse Phospho MCM2 (S53) by IHC. Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) at a dilution of 1:200.
ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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