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Catalog Number | orb1519726 |
---|---|
Category | Antibodies |
Description | MCM2, Phospho (S53) Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | ICC, IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Surrounding Serine 53 |
Concentration | 250 µg/ml |
Dilution range | WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MCM2, Phospho (S53) |
UniProt ID | P49736 |
NCBI | NP_004517.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | D3S3194; MITOTIN; minichromosome maintenance prote Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519726) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Detection of human Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 µg) from HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (orb1519726) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal.
Detection of human Phospho-MCM2 (S53) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MCM2 antibody used for IP. Immunoprecipitates where either treated with phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho-MCM2 (S53) , orb1519726 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Total MCM2.
ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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