mCherry antibody

• Catalog Number: orb66657
• Category: Antibodies
• Description: mCherry antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5A12A7H10
• Tested applications: ELISA, IHC-P, WB
• Isotype: Mouse IgG1
• Immunogen: Human mCherry recombinant protein (orb178365) was used as immunogen.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 1 μg/ml
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Monoclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: mCherry
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-APRF antibody, anti-Acute-phase response fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Shearin, Harold K. et al. Expansion of the gateway multisite recombination cloning toolkit PLoS One, 8, e77724 (2013)

PubMed: 24204935

Applications

IHC

Reactivity

Drosophila

WB analysis of protein 20 ng (lane 1), protein 50 ng (lane 2), protein 100 ng (lane3), protein 200 ng (lane 4) Recombinant protein using mCherry antibody (1 ug/ml)

Western blot analysis of mcherry protein (lane 1) Recombinant protein using anti-mCherry (1 ug/ml)