Mca-RPKPVE-Nval-WR-K(Dnp)-NH2

• Catalog Number: orb1140572
• Category: Proteins
• Description: Fret substrate for MMP-3 and MMP-10; Fluorescent labelled peptides.
• Modifications: 7-methoxycoumarin (Mca) at the N terminal, X is norvaline, Lysine 10 is substuted with a 2, 4-dinitrophenyl (Dnp) group, and the C terminal is amidated.
• Structure: Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2
• Target Areas: Matrix metalloproteases
• Form/Appearance: Freeze dried solid
• Purity: > 95% by HPLC
• MW: 1675.9 Da
• Formula: C₇₈H₁₁₀N₂₂O₂₀
• Solubility (25°C): Soluble in DMSO
• Protein Sequence: Mca-RPKPVEXWRK(Dnp)-NH2
• Storage: Store desiccated, frozen and in the dark
• Alternative names: 2,4-dinitrophenyl (Dnp), 7-methoxycoumarin, MMP-3
• Background: Mca-RPKPVE-Nval-WRK(Dnp)-NH2, also known as Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate, is an excellent substrate for MMP-3 (stromelysin 1) and MMP-10 (stromelysin 2), but not a substrate MMP-1 or MMP-2. Mca-RPKPVE-Nval-WRK(Dnp)-NH2 substrate contains a highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. The cleavage site is the peptide bond between Glu and Nval (norvaline). Mca-RPKPVE-Nval-WRK(Dnp)-NH2 is also an excellent substrate for trypsin, HGF activator and factor Xa.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.