MBP Antibody / Myelin Basic Protein

• Catalog Number: orb699743
• Category: Antibodies
• Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4273 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP. MBP/4273 is also used in a 2-site ELISA with clone MBP/4275. Both are used interchangeably as capture and detection antibody.
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: MBP/4273
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rat IgG2a
• Immunogen: The epitope for this Myelin Basic Protein antibody has been mapped to the 82-87 amino acid region (DENPVV).
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Myelin Basic Protein antibody is available for research use only.
• UniProt ID: P02686
• Storage: Store the Myelin Basic Protein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain with Myelin Basic Protein antibody (clone MBP/4273). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain lysate with Myelin Basic Protein antibody (clone MBP/4273). Isoforms may be visualized from 20~37 kDa.

SDS-PAGE analysis of purified, BSA-free Myelin Basic Protein antibody (clone MBP/4273) as confirmation of integrity and purity.