MBP Antibody / Myelin Basic Protein

• Catalog Number: orb699742
• Category: Antibodies
• Description: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin. MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. MBP/4272 recognizes an epitope in the 119-131 region of MBP, useful in clinical diagnosis to detect MBP levels in human, rat and cow MBP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MBP/4272
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Amino acids GAEGQRPGFGYGG were used as the immunogen for the MBP antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This MBP antibody is available for research use only.
• UniProt ID: P02686
• Storage: Store the MBP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MBP antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain with MBP antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.

Western blot testing of human U-87 MG cell lysate with MBP antibody. Isoforms may be visualized from 20~37 kDa.

SDS-PAGE analysis of purified, BSA-free MBP antibody as confirmation of integrity and purity.