Mast Cell Chymase/CMA1 Antibody

• Catalog Number: orb182387
• Category: Antibodies
• Description: Mast Cell Chymase/CMA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequences identity with mouse and rat CMA1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 27325 MW
• UniProt ID: P23946
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Chymase;3.4.21.39;Alpha-chymase;Mast cell protease
• Note: For research use only
• Application notes: WB: The detection limit for CMA1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CMA1 using anti-CMA1 antibody.Lane 1:human PC-3 cell;2:human HepG2 cell;3:rat liver tissue;4:mouse HEPA1-6 cell.

IHC analysis of CMA1 using anti-CMA1 antibody. CMA1 was detected in a paraffin-embedded section of human placenta tissue.