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Catalog Number | orb1929201 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB0922 |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 48296 Da |
Target | This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human MAPK8. |
UniProt ID | P45983 |
NCBI | NP_620634.1, NP_620637.1, NP_001265476.1, NP_002741.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Mitogen-activated protein kinase 8, MAP kinase 8, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of MAPK8 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
All lanes: Anti-MAPK8 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
MAPK8 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK8 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.