MAP3K5 (Phospho-S966) antibody

• Catalog Number: orb393099
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MAP3K5 (Phospho-S966)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S966 of human ASK1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MAP3K5
• Entrez: 26408, 4217
• UniProt ID: O35099, Q99683
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ASK1; MAPKKK5; MEKK5; Mitogen-activated protein ki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ASK1 (Phospho-S966) expression in HEK293T TNFa-treated (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 154 kD; Observed band size: 155 kD)

Immunohistochemical analysis of ASK1 (Phospho-S966) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.