MAP2K2 Antibody

• Catalog Number: orb1262838
• Category: Antibodies
• Description: MAP2K2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Drosophila, Gallus, Hamster, Mouse, Rabbit, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: MAP2K2
• UniProt ID: P36507
• NCBI: P36507
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Dual specificity mitogen-activated protein kinase
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35 ug/lane)

Western blot analysis of MAP2K2 using rabbit polyclonal MAP2K2-S222 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.