Mammaglobin A Antibody / SCGB2A2

• Catalog Number: orb606912
• Category: Antibodies
• Description: Mammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MGB/2682R
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant Mammaglobin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Mammaglobin antibody is available for research use only.
• UniProt ID: Q13296
• Storage: Store the recombinant Mammaglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Mammaglobin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human breast carcinoma stained with recombinant Mammaglobin antibody (clone MGB/2682R). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human HeLa, MCF-7, SK-Br3 and T47D cell lysate with recombinant Mammaglobin antibody (clone MGB/2682R). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

SDS-PAGE analysis of purified, BSA-free recombinant Mammaglobin antibody (clone MGB/2682R) as confirmation of integrity and purity.

Immunofluorescent staining of permeabilized human MCF7 cells with recombinant Mammaglobin antibody (clone MGB/2682R, green) and Reddot nuclear stain (red).