Mammaglobin A Antibody / SCGB2A2

• Catalog Number: orb606602
• Category: Antibodies
• Description: Mammaglobin, also called Secretoglobin family 2A member 2, or SCGB2A2, is a 93 amino acid glycoprotein with homology to other secretoglobin-uteroglobin family members. It was originally identified as a breast cancer restricted biomarker by differential screening. Mammaglobin related to secretoglobin family that includes human uteroglobin and lipophilin. Mammaglobin antibody stains cytoplasm of normal breast epithelial cells as well as primary and metastatic breast carcinomas. Mammaglobin expression is absent in prostate, kidney, colon, rectum, small intestine, stomach, pancreas, lung, and thyroid tissues. Mammaglobin may be used as part of an immunohistochemical panel for determination of metastatic breast carcinoma and tumor of unknown primary origin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MGB/2000
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the Mammaglobin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Mammaglobin antibody is available for research use only.
• UniProt ID: Q13296
• Storage: Store the Mammaglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Mammaglobin antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human breast carcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human SK-BR-3 cell lysate (mammary gland tumor cell line) with Mammaglobin antibody (clone MGB/2000). Expected molecular weight: 10-21 kDa depending on level of glycosylation.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Mammaglobin antibody (clone MGB/2000). These results demonstrate the foremost specificity of the MGB/2000 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Mammaglobin antibody (clone MGB/2000) as confirmation of integrity and purity.

IHC testing of FFPE human breast adenocarcinoma stained with Mammaglobin antibody (MGB/2000). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.