MAD2L2 antibody

• Catalog Number: orb345442
• Category: Antibodies
• Description: MAD2L2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human MAD2L2.
• Concentration: 0.68 mg/mL
• Dilution range: ELISA: 1:7,500 - 1:30,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
• Conjugation: Unconjugated
• UniProt ID: Q9UI95
• NCBI: 187960072
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-MAD2L2 antibody, MAD2B, REV7, hREV7, R
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. For western blotting HeLa whole cell lysates and nuclear extracts are suggested. Reactivity in other immunoassays is unknown. Antibody activity can be blocked using a control peptide (000-001-470).
• Expiration Date: 12 months from date of receipt.

Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Lane 1: HeLa whole cell lysate (p/n orb348668) and nuclear lysate (p/n orb348671) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.

Biorbyt's Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.