Mad2L1 Antibody

• Catalog Number: orb234328
• Category: Antibodies
• Description: Mad2L1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23510 MW
• UniProt ID: Q13257
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mitotic spindle assembly checkpoint protein MAD2A;
• Note: For research use only
• Application notes: WB: The detection limit for Mad2L1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Mad2L1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Western blot analysis using Anti-Mad2L1 Picoband antibody.Lane 1:293T Cell;2:COLO320 Cell.

IF analysis of Mad2L1 using anti-Mad2L1 antibody. Mad2L1 was detected in immunocytochemical section of A431 cells.