MAD1/MAD1L1 Antibody

• Catalog Number: orb234308
• Category: Antibodies
• Description: MAD1/MAD1L1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MAD1 recombinant protein (Position: L362-A632). Human MAD1 shares 81% amino acid (aa) sequence identity with mouse MAD1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 83067 MW
• UniProt ID: Q9Y6D9
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Mitotic spindle assembly checkpoint protein MAD1;M
• Note: For research use only
• Application notes: WB: The detection limit for MAD1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-MAD1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of MAD1 using anti-MAD1 antibody.Lane 1:human HeLa cell;2:human Raji cell;3:human Jurkat cell;4:human HepG2 cell.5:human HL-60 cell.6:human THP-1 cell.7:human Caco-2 cell.

WB analysis of MAD1 using anti-MAD1 antibody.Lane 1:rat brain tissue;2:rat kidney tissue;3:rat lung tissue;4:rat liver tissue;5:mouse brain tissue;6:mouse kidney tissue;7:mouse lung tissue;8:mouse liver tissue;9:mouse NIH3T3 cell.

IF analysis of MAD1 using anti-MAD1 antibody. MAD1 was detected in immunocytochemical section of U20S cells.

IHC analysis of MAD1 using anti-MAD1 antibody.MAD1 was detected in paraffin-embedded section of mouse intestine tissues.

IHC analysis of MAD1 using anti-MAD1 antibody.MAD1 was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of MAD1 using anti-MAD1 antibody.MAD1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of MAD1 using anti-MAD1 antibody.MAD1 was detected in immunocytochemical section of HeLa cell.