Macrophages F4/80 antibody (FITC)

• Catalog Number: orb1109132
• Category: Antibodies
• Description: Mouse monoclonal antibody against Macrophages F4/80 conjugated to FITC
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IHC-Fr, PS, WB
• Reactivity: Mouse
• Isotype: Rat IgG2a
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified FITC conjugated antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
• Conjugation: FITC
• Storage: Product should be stored at 4degC. Under recommended storage conditions, product is stable for one year.
• Note: For research use only
• Application notes: W: Mouse bone-marrow derived macrophages; non-reduced; ~125 kDa (Ref 1); reduction with 2-mercaptoethanol destroys BM8 antigen. F: tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control. P: fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control (Ref 3). FC: fixed with 1 % paraformaldehyde (Ref 1).
• Expiration Date: 12 months from date of receipt.