M Mlkl Antibody (C-term)

• Catalog Number: orb1935968
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34842
• Tested applications: IHC-P, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Dilution range: IHC-P: 1~400, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54317 Da
• Target: This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
• UniProt ID: Q9D2Y4
• NCBI: NP_083281.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mixed lineage kinase domain-like protein, Mlkl {EC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunohistochemical analysis of paraffin-embedded Human tonsil section using M Mlkl antibody. Diluted at 1~400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-M Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 53KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse testis lysates. Lane 2: mouse liver lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse lung lysates. Lane 2: NIH/3T3 whole cell lysates. Lane 3: mouse liver lysates. Lane 4: mouse testis whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.