Lysozyme LYZ Rabbit Monoclonal Antibody

• Catalog Number: orb547865
• Category: Antibodies
• Description: Lysozyme LYZ Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: EBO-12
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Lysozyme
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 16537 MW
• UniProt ID: P61626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Lysozyme C;3.2.1.17;1,4-beta-N-acetylmuramidase C;
• Note: For research use only
• Application notes: WB 1:5000-1:10000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:20
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded mouse kidney, using Lysozyme Antibody.

Western blot analysis of Lysozyme using anti-Lysozyme antibody (orb547865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Lysozyme antigen affinity purified monoclonal antibody (Catalog # orb547865) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for Lysozyme at approximately 15 kDa. The expected band size for Lysozyme is at 17 kDa.