Lysozyme C Antibody / LYZ

• Catalog Number: orb2637393
• Category: Antibodies
• Description: Lysozyme is an enzyme, commonly referred to as the bodys own antibiotic since it kills bacteria. Natural substrate of lysozyme c is the bacterial cell wall peptidoglycan (cleaving the beta[1-4] glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme c is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Lysozyme C is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. This antibody labels myeloid cells, histiocytes, granulocytes, macrophages and monocytes. It is helpful in the identification of myeloid or monocytic nature of acute leukemia.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LYZ/3942
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 18-147 was used as the immunogen for the LYZ antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This LYZ antibody is available for research use only.
• UniProt ID: P61626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the LYZ antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen tissue with LYZ antibody (clone LYZ/3942). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with LYZ antibody (clone LYZ/3942). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human spleen tissue and HepG2 cell lysates using LYZ antibody (clone LYZ/3942).

SDS-PAGE analysis of purified, BSA-free LYZ antibody (LYZ/3942) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using LYZ antibody (clone LYZ/3942). These results demonstrate the foremost specificity of the LYZ/3942 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.