Lysozyme antibody (Peroxidase)

• Catalog Number: orb344904
• Category: Antibodies
• Description: Lysozyme antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Lysozyme Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]. Cross reactivity against Lysozyme from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P00698
• NCBI: NP_990612.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Lysozyme Antibody Peroxidase Conjugati
• Note: For research use only
• Application notes: Anti-Lysozyme Peroxidase Conjugate Antibody has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Dylight 549 conjugated secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: ~14-16 kDa for Lysozyme. Other band(s): none.