Lysozyme antibody (Peroxidase)

• Catalog Number: orb344903
• Category: Antibodies
• Description: Lysozyme antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Lysozyme Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]. Cross reactivity against Lysozyme from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P00698
• NCBI: NP_990612.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Lysozyme Antibody Peroxidase Conjugati
• Note: For research use only
• Application notes: Anti-Lysozyme Peroxidase Conjugate Antibody has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Dylight 549 conjugated secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: ~14-16 kDa for Lysozyme. Other band(s): none.