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Catalog Number | orb344574 |
---|---|
Category | Antibodies |
Description | Lysozyme antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, IP, WB |
Reactivity | Gallus |
Isotype | IgG |
Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme. |
Concentration | 1.0 mg/ml |
Dilution range | ELISA: 1:25,000, IHC: 1:500 - 1:2,000, IP: 1:100, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This purified antibody is directed against lysozyme from hen egg white protein. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined. |
Conjugation | Unconjugated |
UniProt ID | P00698 |
NCBI | NP_990612.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmu Read more... |
Note | For research use only |
Application notes | Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
ELISA Results of Rabbit Anti-Lysosome Antibody. Each well was coated in duplicate with 1.0 µg of Lysosome. The working dilution is 1:5000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).
Western Blot of Rabbit anti-Lysozyme (Hen Egg White) Antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Primary antibody: Rabbit anti-Lysozyme (Hen Egg White) Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: ~14-16 kDa for Lysozyme (Hen Egg White).
Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Dylight 549 conjugated secondary antibody at 1:10000 for 1.5 hrs at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 16 kDa for Lysozyme. Other band(s): none.
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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