Lysine Acetylated Antibody Biotin Conjugated

• Catalog Number: orb420484
• Category: Antibodies
• Description: Lysine Acetylated antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Dilution range: ELISA: 1:5,000, WB: 1:1,000-1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-AcK was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin. Reactivity is specific for acetylated lysine.
• Conjugation: Biotin
• Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: None
• Alternative names: acetyl Lysine antibody, Acetylated lysine antibody
• Note: For research use only
• Application notes: This affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with acetylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
• Expiration Date: 12 months from date of receipt.

Biorbyt's Affinity Purified anti-Acetylated Lysine (AcK) antibody is shown to detect acetylated histone in TSA-treated mouse spleen cell lysate (Panel A); control (left lane) and TSA-treated mouse spleen cell lysate (right lane) in panel B; and in acetylated BSA loaded as indicated (panel C).