Luciferase Antibody

• Catalog Number: orb750438
• Category: Antibodies
• Description: luxA antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, IP, WB
• Isotype: Antiserum
• Immunogen: Luciferase [Photobacterium fischerii]
• Antibody Type: Primary Antibody
• Concentration: 75 mg/ml
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii]. Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P19907
• NCBI: P29238.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Luciferase Antibody, Alkanal monooxyge
• Note: For research use only
• Application notes: Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1 ug/ml 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP orb347654 at 1:40000 for 30 min RT.