Luciferase Antibody

• Catalog Number: orb750393
• Category: Antibodies
• Description: Luciferase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Isotype: Antiserum
• Immunogen: Luciferase [Firefly]
• Antibody Type: Primary Antibody
• Concentration: 82 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Luciferase [Firefly]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti-Luciferase Antibody, Luciferase Firefly,
• Note: For research use only
• Application notes: Anti-Luciferase Antibody has been tested by western blot and is suitable in ELISA. This product can be assayed against 1.0 ug of Luciferase [Firefly] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Luciferase Antibody. Lane 1: Luciferase (Firefly). Load: 50 ng per lane. Primary antibody: Goat anti-Luciferase Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP goat secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Luciferase.