Luciferase antibody

• Catalog Number: orb344219
• Category: Antibodies
• Description: Luciferase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Isotype: IgG
• Immunogen: Luciferase [Photinus pyralis (Firefly)]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:200 - 1:1000, IHC: 1:1500, IF: User Optimized, WB: 1:1000 - 1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Luciferase Antibody, Luciferin 4-monooxy
• Note: For research use only
• Application notes: Anti-Luciferase Antibody has been tested in Western Blot, IHC, IF, and ELISA. Expect a band ~60kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Goat Anti-Luciferase Antibody. Each well was coated in duplicate with 1.0 µg of Luciferase [Firefly]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:6050. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n orb348651.

Western Blot of Goat anti-Luciferase antibody. Lane 1 Marker: Opal Pre-stained ladder. Lane 2: HEK293 lysate (p/n orb348669). Lane 3: HeLa Lysate (p/n orb348668). Lane 4: CHO/K1 Lysate. Lane 5: E-coli HCP Control (p/n orb342262). Lane 6: J774A.1 Lysate. Lane 7: C2C12 Lysate (p/n orb348725). Lane 8: Mouse Embryonic Fibroblast Lysate (p/n orb348722). Lane 9: NIH/3T3 Lysate (p/n orb348714). Lane 10: Mouse Liver Lysate (p/n orb348718). Lane 11: PC-12 Lysate (p/n orb348733). Lane 12: Rat Brain Lysate (p/n orb348727). Lane 13: Rat Testis Lysate. Lane 14: Luciferase [Photinus pyralis (Firefly)]. Lane 15 Marker: Opal Pre-stained ladder. Load: 10 µg of lysate or 50 ng of purified protein per lane. Primary antibody: Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase Goat secondary antibody (p/n orb347032) at 1:40000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: ~60 kDa for Luciferase.

Western Blot of Goat anti-Luciferase Antibody. Lane 1: Luciferase. Load: 50 ng per lane. Primary antibody: Luciferase antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: (p/n orb348637) for 30 min at RT. Predicted/Observed size: ~60 kDa for Luciferase. Other band(s): None.