Llama IgG2 isotype control Biotin Antibody

• Catalog Number: orb343843
• Category: Proteins
• Description: Llama IgG2 isotype control Biotin Antibody
• Tested applications: DOT, ELISA, FC, WB
• Isotype: IgG
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to unconjugated llama IgG1 or IgG3 are observed. Biotin Conjugated Llama IgG2 was proven by Dot Blot.
• Conjugation: Biotin
• Source: Llama
• Biological Origin: Llama
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Llama IgG2 isotype, Llama IgG2 subclass isotype, L
• Note: For research use only
• Application notes: Llama IgG2 Biotin conjugated isotype control has been tested by dot blot and can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Llama IgG2 Isotype control Biotin Conjugated. Llama IgG2 Isotype control Biotin Conjugate (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23. Antibody: Streptavidin (p/n orb348765) at 1:40000 for 30 mins at RT. Block: BlockOut (p/n orb348644) for 30 mins at RT. Exposure: 1 sec.