LITAF Antibody

• Catalog Number: orb1246701
• Category: Antibodies
• Description: LITAF Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PDGKGMNPPSYYTQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 25kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 6.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: LITAF
• UniProt ID: Q99732
• NCBI: NP_001129945.1, NP_004853.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: LITAF, lipopolysaccharide-induced TNF factor, CMT1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 25kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 6.5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246701 (6.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.