LIGHT Detection Set (Risk Free)

• Catalog Number: orb1227440
• Category: Assays and Kits
• Description: LIGHT Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: LIGHT antibodies were raised against the extracellular domain of human LIGHT.
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Mouse Monoclonal: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Titration curve analysis of LIGHT mAbs to detect recombinant LIGHT in ELISA with orb1239555, orb1239558, orb1239554, and orb1239565 abs at decreasing concentrations.

Western blot analysis of LIGHT in overexpressing HEK293 cells using orb1239555, orb1239558, orb1239554, and orb1239565 antibody at 0.5 and 1 μg/ml, respectively.

Immunocytochemistry of LIGHT in overexpressing HEK293 cells using (A) orb1239555, (B) orb1239558, (C) orb1239554, (D) orb1239565, and (E) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of LIGHT in overexpressing HEK293 cells using (A) orb1239555, (B) orb1239558, (C) orb1239554, (D) orb1239565, and (E) control mouse IgG antibody at 2 μg/ml.

Immunofluorescence of LIGHT in human lymphoma tissue using using (A) orb1239555, (B) orb1239558, (C) orb1239554, (D) orb1239565, and (E) control mouse IgG antibody at 20 μg/ml.

Immunohistochemistry of LIGHT in human lymphoma tissue using using (A) orb1239555, (B) orb1239558, (C) orb1239554, (D) orb1239565, and (E) control mouse IgG antibody at 5 μg/ml.

Flow cytometry analysis of LIGHT overexpressing HEK293 cells using (A) orb1239555, (B) orb1239558, (C) orb1239554, (D) orb1239565, and (E) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.