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Catalog Number | orb1258418 |
---|---|
Category | Antibodies |
Description | LIG1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 140kDa |
Target | LIG1 |
UniProt ID | P18858 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | LIG1, MGC117397, MGC130025, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using LIG1 antibody (orb1258418) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using LIG1 antibody (orb1258418). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using LIG1 antibody (orb1258418).
ICC, IHC-Fr, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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